Senior researcher
ales.tichy@unob.cz
ORCID: 0000-0002-8262-5703
Aleš earned his PhD In 2008, at Faculty of Medicine of Charles University in Hradec Králové with a thesis entitled "Apoptosis and proliferation of tumor cell lines after radiation and drug treatment." Since then, dedicated to cell signaling, he systematically addresses the molecular mechanisms of DNA damage in relation to the effects of ionizing radiation. His knowledge is applied in radio-sensitization of tumor cell via specific inhibition of signaling pathways and to biological dosimetry. He employs both conventional immuno-detection techniques of molecular biology and modern approach of mass spectrometry together with quantitative phosphoproteomics. In 2013 he defended his work "Repair of radiation damage and its importance in radio-sensitization of the cancer cells." and was appointed Associated professor.
| Assoc. prof. | (2013) | Radiobiology, Faculty of Military Health Sciences in Hradec Králové, University of Defence |
| PharmDr. | (2009) | Pathobiochemistry and Xenobiochemistry, Faculty of Pharmacy in Hradec Králové, Charles University in Prague |
| PhD. | (2008) | Medical Chemistry and Biochemistry, Faculty of medicine in Hradec Králové, Charles University in Prague |
| MSc. | (2003) | General Pharmacy, Faculty of Pharmacy in Hradec Králové, Charles University in Prague |
• Ionizing radiation, biodosimetry
• Radiation-induced cell signaling, DNA repair, apoptosis
• Radio-sensitization of tumor cells
• Quantitative phosphoproteomics, mass spectrometry, immunodetection techniques
• General Pharmacy
| 2004–2008 | Lecturer, Institute of Medical Biochemistry, Faculty of Medicine in Hradec Králové, Charles University in Prague |
| 2005–2012 | Lecturer, Department of Radiobiology, Faculty of Military Health Sciences in Hradec Králové, University of Defence in Brno |
| 2012–present | Assistant Professor, Department of Radiobiology, Faculty of Military Health Sciences in Hradec Králové, University of Defence in Brno |
2003 Department of Biochemistry, Faculty of Pharmacy, University of Porto, Porto, Portugal
Focus: protein detection methods, Western-blot
2005 Institute of Molecular and Cellular Biology, Porto, Portugal
Focus: 2-dimensional electrophoresis
2010 National Center for Glycomics and Glycoproteomics, University of Indiana, Bloomington, Indiana, USA
Focus: Fosfoproteomika, mass spectrometry (LT-QFT, MALDI-TOF/TOF)
2010 NATO School Oberammergau, Germany - NBC Analysis Operator Course P3-74-B
Focus: CBRN issues, NBC Analysis software
2005–2006 Czech Medical Society
2006–present Czech Society for Radiobiology and crisis planning CSL JEP
2010–present European Radiation Research Society (ERRS)
2010–present American Society for Mass Spectrometry (ASMS)
2011–2012 Joint Assessment Team CBRN - NATO Responce Force
2011–present Departmental Council for Military radiobiology FVZ UO
Title: BIOMIT: Biodosimetry using human blood lymphocytes mitochondrial DNA alterations.
Funding: National Institutes of Health USA
Duration: 2014-2015
Role: Principal investigator
Title: PANILE: Phosphoproteomic Analisys of Irradiated Leukemic cells
Funding: SCIEX - Swiss-Czech Scientific Exchange Programme
Duration: 2015
Role: supervisor, co-worker
Title: Phosphoproteomic analysis of leukemic cell lines after ionizing radiation
Funding: Grant Agency of the Czech Republic - P206/12/338
Duration: 2012-2014
Role: Principal investigator
Title: Radio-sensitizing effect of inhibition of DNA repair in MOLT-4 and SAOS-2 cells: Phosphoproteomic analysis of irradiated tumor cells.
Funding: Grant Agency of Charles University – 1220313
Duration: 2013-2014
Role: supervisor, co-worker
Title: Modulation of cellular response of normal fibroblasts to irradiation: the importance of radioprotection
Funding: Faculty of Military Health Sciences
Duration: 2013-2015
Role: Supervisor
• Post-graduate - 4x mentor (Šalovská MS, Ďurišová MS, Ondrej MS, Čecháková MS), 1x mentor-specialist (Novotná MS)
• Pre-graduate - 2x mentor (successfully defended - Sedlaříková BC, Ďurišová BC)
• lecturer at FVZ UO (Military Radiobiology), FSZV UP (Biophysics), LF HK University (Medical Biochemistry)
2004 3rd place at the conference PGS students - New Frontiers in PhD studies - Hradec Králové
2014 distinguished Plaquette of Rector - University of Defence
Publications: 18x first or corresponding author; 57x total
Citation: 429 (358 excluding self-citations)
H-index: 12
(dated 04/2020)
2020
Filipova A, Marek J, Havelek R, Pejchal J, Jelicova M, Cizkova J, Majorosova M, Muckova L, Kucera T, Prchal L, Psotka M, Zivna N, Koutova D, Sinkorova Z, Rezacova M, Tichy A. Substituted Piperazines as Novel Potential Radioprotective Agents. Molecules. 2020;25(3):532. Published 2020 Jan 25. doi:10.3390/molecules25030532
Kultova G, Tichy A, Rehulkova H, Myslivcova-Fucikova A. The hunt for radiation biomarkers: current situation. Int J Radiat Biol. 2020;96(3):370–382. doi:10.1080/09553002.2020.1704909
Marek J, Tichy A, Havelek R, et al. A novel class of small molecule inhibitors with radioprotective properties. Eur J Med Chem. 2020;187:111606. doi:10.1016/j.ejmech.2019.111606
2019
Cechakova L, Ondrej M, Pavlik V, Jost P, Cizkova D, Bezrouk A, Pejchal J, Amaravadi RK, Winkler JD, Tichy A. A Potent Autophagy Inhibitor (Lys05) Enhances the Impact of Ionizing Radiation on Human Lung Cancer Cells H1299. Int J Mol Sci. 2019;20(23):5881. Published 2019 Nov 23. doi:10.3390/ijms20235881
Šinkorová Z, Filipová A, Vávrová J, et al. Investigation of the radioprotective effect of orthovanadate in mice after total body irradiation. Radiat Prot Dosimetry. 2019;186(2-3):149–154. doi:10.1093/rpd/ncz192
2018
Tichy A, Kabacik S, O'Brien G, Pejchal J, Sinkorova Z, Kmochova A, Sirak I, Malkova A, Beltran CG, Gonzalez JR, Grepl J, Majewski M, Ainsbury E, Zarybnicka L, Vachelova J, Zavrelova A, Davidkova M, Markova Stastna M, Abend M, Pernot E, Cardis E, Badie C. The first in vivo multiparametric comparison of different radiation exposure biomarkers in human blood. PLoS One. 2018 Feb 23;13(2):e0193412. doi: 10.1371/journal.pone.0193412. IF = 2,806
O'Brien G, Cruz-Garcia L, Majewski M, Grepl J, Abend M, Port M, Tichy A, Sirak I, Malkova A, Donovan E, Gothard L, Boyle S, Somaiah N, Ainsbury E, Ponge L, Slosarek K, Miszczyk L, Widlak P, Green E, Patel N, Kudari M, Gleeson F, Vinnikov V, Starenkiy V, Artiukh S, Vasyliev L, Zaman A, Badie C. FDXR is a biomarker of radiation exposure in vivo. Sci Rep. 2018 Jan 12;8(1):684. doi: 10.1038/s41598-017-19043-w. IF = 4,259
Manning G, Tichý A, Sirák I, Badie C. Radiotherapy-Associated Long-term Modification of Expression of the Inflammatory Biomarker Genes ARG1, BCL2L1, and MYC. Front Immunol. 2017 Apr 10;8:412. doi: 10.3389/fimmu.2017.00412. eCollection 2017. IF = 6,429.
Tichy A, Marek J, Havelek R, Pejchal J, Seifrtova M, Zarybnicka L, Filipova A, Rezacova M, Sinkorova Z. New light on an old friend: targeting PUMA in radioprotection and therapy of cardiovascular and neurodegenerative diseases. Curr Drug Targets. 2018 doi: 10.2174/1389450119666180406110743. IF = 3,259
2017
H. Dörr, M. Abend, W. F. Blakely, D. L. Bolduc, D. Boozer, T. Costeira, T. Dant, A. De Amicis, S. De Sanctis, M. Dondey, M. Drouet, F. Entine, S. Francois, G. Gagna, N. Guitard, F. Hérodin, M. Hoefer, A. Lamkowski, G. La Sala, F. Lista, P. Loiacono, M. Majewski, P. Martigne, D. Métivier, X. Michel, J. Pateux, J. Pejchal, G. Reeves, D. Riccobono, Z. Sinkorova, L. Soyez, D. Stricklin, A. Tichy, M. Valente, C. R. Woodruff Jr., L. Zarybnicka and M. Port. Using clinical signs and symptoms for medical management of radiation casualties-2015 NATO exercise. Radiation ResearchVolume 187, Issue 3, 1 March 2017, Pages 273-286. IF = 2,539
2016
Pejchal J, Sinkorova Z, Tichy A, Pruchova S, Kmochova A, Durisova K, Cechakova L, Lierova A, Ondrej M, Nemcova M, Kubelkova K, Fatorova I, Bures J, Tacheci I, Kuca K, Vavrova J. Epidermal Growth Factor Attenuates Delayed Ionizing Radiation-Induced Tissue Damage in Bone Marrow Transplanted Mice. Radiat Res. 2016 Sep;186(3):264-74. doi: 10.1667/RR14247.1. IF = 2,539
Ondrej M, Cechakova L, Durisova K, Pejchal J, Tichý A*. To live or let die: unclear task of autophagy in the radiosensitization battle. Rad Oncol. 2016; 119:265-75. IF = 4,817
2015
Ďurišová K, Šalovská B, Pejchal J, Tichý A*. Chemical inhibition of DNA repair kinases as a promising tool in oncology. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015 Oct 21. [Epub ahead of print] IF = 1,200
2014
Tichý A, Ďurišová K, Novotná E, Šalovská B, Pejchal J, Zárybnická L, Vávrová J, Dye N, Šinkorová Z. Radio-sensitization of human leukaemic MOLT-4 cells by DNA-dependent protein kinase inhibitor, NU7441. Radiat Environ Biophysics. 2014; 53: 83-92. IF = 1,754
Šalovská B, Fabrik I, Ďurišová K, Link M, Vávrová J, Řezáčová M, Tichý A*. Radiosensitization of Human Leukemic HL-60 Cells by ATR Kinase Inhibitor (VE-821): Phosphoproteomic Analysis. Int J Mol Sci 2014; 15: 12007-26. IF = 2,464
2013
Zárybnická L, Vávrová J, Havelek R, Tichý A, Pejchal J, Šinkorová Z. Lymphocyte subsets and their H2AX phosphorylation in response to in vivo irradiation in rats. Int J Rad Biol 2013; 89: 110-7. IF = 2,178
Novotná E, Pejchal J, Lukášová E, Šalovská B, Vávrová J, Tichý A* .DNA-dependent protein kinase and its inhibition in support of radiotherapy. Int J Rad Biol 2013; 89: 416-423. Review. IF = 2,275
2012
Pejchal J, Novotný J, Mařák V, Osterreicher J, Tichý A, Vávrová J, Sinkorová Z, Zárybnická L, Novotná E, Chládek J, Babicová A, Kuča K. Activation of p38 MAPK and expression of TGF-β1 in rat colon enterocytes after whole body γ-irradiation. Int J Radiat Biol. 2012; 4: 348-58. IF = 2,275.
2011
Tichý A, Šalovská B, Řehulka P, Klimentová J, Vávrová J, Stulík J, Hernychová L. Phosphoproteomics: searching for a needle in a haystack. J Proteome 2011 74: 2786-97. IF = 5,074
Tichy A, Muthna D, Vávrová J, Pejchal J, Sinkorova Z, Zarybnicka L, Rezacova M. Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic MOLT-4 cells. J Appl Biomed 2011; 9: 1-8. IF = 1,933
2010
Vavrova J, Tichy A, Rezacova M, Zarybnicka L, Sinkorova Z, Osterreicher J. Exposure to fractionated dose of 60 Gy changes molecular response of HL-60 cells to irradiation. Gen Physiol Biophys 2010; 29: 275-81.IF = 1,146
2009
Tichý A, Záskodová D, Zoelzer F, Vávrová J, Sinkorová Z, Pejchal J, Osterreicher J, Rezácová M. Gamma-radiation-induced phosphorylation of p53 on serine 15 is dose-dependent in MOLT-4 leukaemia cells. Folia Biol (Praha) 2009; 55: 41-4. IF = 0,924
Pejchal J, Osterreicher J, Kassa J, Tichy A, Micuda S, Sinkorova Z, Zarybnicka L. Soman poisoning alters p38 MAPK pathway in rat cerebellar Purkinje cells. J Appl Toxicol 2009; 29: 338-45. IF = 2,073
2008
Tichý A, Záškodová D, Pejchal J, Řezáčová M, Österreicher J, Vávrová J, Cerman J. Gamma irradiation of human leukaemic cells HL-60 and MOLT-4 induces decrease in Mcl-1 and Bid, release of cytochrome c, and activation of caspase-8 and caspase-9. Int J Rad Biol 2008; 84: 523-30. IF = 2,178
