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Rudolf received his Ph.D. in 2019 in the study program Analytical Chemistry at the Faculty of Chemical Technology, University of Pardubice. His thesis was focused on the development and utilization of various types of materials suitable for isolation and analysis of clinically important proteins. Within his Ph.D. studies he also utilized mass spectrometry techniques for analysis of proteins and their posttranslational modifications. Currently, he is employed as postdoctoral fellow at Biomedical Research Centre of University Hospital Hradec Králové. He deals with application of mass spectrometry-based methods for analysis of clinically relevant samples.
Faculty of Chemical Technology, University of Pardubice, Study program Analytical chemistry
Disertation thesis: New materials and techniques for separation and analysis of clinically important proteins
Faculty of Chemical Technology, University of Pardubice, Study program Analysis of Biological MaterialsDiploma thesis: New methodical approaches in specific isolation of phosphoproteins and phosphopeptides
Postdoctoral fellow, Biomedical Research Centre, University Hospital Hradec Králové
Junior researcher, Faculty of Chemical Technology, University of Pardubice
|10/2016-12/2016||3 months research internship at the Vienna University of Technology, Institute of Chemical Technologies and Analytics (Vienna, Austria)|
Students: Bachelor thesis: 1 (head of work)
Diploma thesis: 9 (consultant)
Best poster + short talk – 8th Conference of CSMS, Olomouc, Czech Republic, 2019
Kupcik, R., Macak, J. M., Rehulkova, H., Sopha, H., Fabrik, I., Anitha, V.C., Klimentova, J., Murasova, P., Bilkova, Z., Rehulka, P., Amorphous TiO2 Nanotubes as a Platform for Highly Selective Phosphopeptide Enrichment. ACS OMEGA 2019, 4, 12156-12166. (IF: 2.584)
Kročová, E., Neradová, S., Kupcik, R., Janovská, S., Bílková, Z., Heidingsfeld, O., PHO15 genes of Candida albicans and Candida parapsilosis encode HAD-type phosphatases dephosphorylating 2-phosphoglycolate. FEMS Yeast Research 2019, foy112. (IF: 2.609)
Hromadkova, L., Kupcik, R., Vajrychova, M., Prikryl, P., Charvatova, A., Jankovicova, B., Ripova, D., Bilkova, Z., Slovakova, M., Kinase-loaded magnetic beads for sequential in vitro phosphorylation of peptides and proteins. The Analyst 2018, 143, 466–474. (IF: 3.864)
Kupcik, R., Rehulka, P., Bilkova, Z., Sopha, H., Macak, J. M., New Interface for Purification of Proteins: One-Dimensional TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles. ACS Applied Materials & Interfaces 2017, 9, 28233–28242. (IF: 8.097)
Hromadkova, L., Kupcik, R., Jankovicova, B., Rousar, T., Ripova, D., Bilkova, Z., Difficulties associated with the structural analysis of proteins susceptible to form aggregates: The case of Tau protein as a biomarker of Alzheimer’s disease. Journal of Separation Science 2016, 39, 799–807. (IF: 2.557)
Kupčík, R., Zelená, M., Řehulka, P., Bílková, Z., Česlová, L., Selective isolation of hydrophobin SC3 by solid-phase extraction with polytetrafluoroethylene microparticles and subsequent mass spectrometric analysis. Journal of Separation Science 2016, 39, 717–724. (IF: 2.557)
Slováková, M., Sedlák, M., Křížková, B., Kupčík, R., Bulánek, R., Korecká, L., Drašar, Č., Bílková, Z., Application of trypsin Fe3O4@SiO2 core/shell nanoparticles for protein digestion. Process Biochemistry 2015, 50, 2088–2098. (IF: 2.529)
Macák M., Kupčík R., Bílková Z. (University of Pardubice), Method for preparation of iron oxide-based magnetic composite carrier for separation of biomolecules.
CZ patent 307934 (28. 08. 2019)
Kupčík R., Macák J., Řehulka P., Bílková Z. (University of Pardubice), Method for separation of biopolymer molecules and a carrier for application of this method.
CZ patent 305599 (18. 11. 2015); WO2016015690 (04. 02. 2016)