NT13599 - Characterization of the diagnostic potential of native polypeptides in amniotic fluid
Provider:
Ministry of Health
Investigator:
Juraj Lenčo, Marian Kacerovský, Vojtěch Tambor, Jana Žďárová Karasová
Abstract:
Preterm premature rupture of membranes (PPROM) is a principal cause of preterm births and considerably increases perinatal morbidity and mortality. The main reason is the causal link with intraamniotic infection (IAI), which occurs in 40-60% of PPROM patients. The most serious cases of IAI may result in fetal inflammatory response (FIRS) and cause permanent health consequences for the newborn or end up by the death of the fetus. FIRS is very often subclinical and thus frequently remains undetected. Currently, there is no tool for its precise prenatal diagnostics available. The project is focused on characterization of the diagnostic potential of native amniotic fluid polypeptides with regard to detection of IAI and FIRS.
Project duration:
1-April-2012 - 31-Dec-2015
NT14150 - Modulation of Colorectal Carcinoma by Biological Therapy - In Vitro Study
Provider:
Ministry of Health
Investigator:
Aleš Ryška, Stanislav John
Proteomics team - Biomedical Research Center
Abstract:
Resecated tumours from appropriate patients with colorectal carcinoma will be histopathologically examined and from the part of the tumour cancer cells will be obtained. First, the tumor cells will be cultured and the prepared cell cultures will be treated by bevacizumab and cetuximab. Subsequently, we will evaluate the changes in the phenotype of primary colorectal cancer tumor lines after stimulation with these agents in order to describe effect on the biological profile of the cells. Samples of these cell lines will be deeply frozen for the further proteomic analysis. Prospectively, in the second phase we would try to identify differences in proteome among the cell lines from patients who responded to the treatment by the particular drug from those where the treatment failed. As a result we would detect the differences both biological both proteomic which may be relevant for the future optimization and individualization of therapy.
Project duration:
1-May-2013 - 31-Dec-2015
Provider:
Ministry of Health
Investigator:
Marian Kacerovský, Ctirad Andrýs, Martin Procházka, Ondřej Šimetka, Petr Janků
Abstract:
Women with preterm prelabor rupture of membranes between gestational age 24 and 36 weeks will be included into the multicentre study. The intensity of inflammatory response (based on levels of cytokines and alarmins) and microbial load of Ureaplasma spp. will be determined in the cervical fluid. The placenta will be assessed for the signs of histological chorioamnionitis and funisitis after delivery. The inflammatory response in the cervical fluid will be characterized with respect to the presence of histological chorioamnionitis and funisitis with an ultimate goal to create a prediction model for these inflammatory complications. The relationship between microbial load of Ureaplasma spp. in cervical fluid and inflammatory response will be evaluated as well. Last goal will be to analyse the potential of microbial load of Ureaplasma spp. for the prediction of histological chorioamnionitis and funisitis.
Project duration:
1-May-2013 - 31-December-2015
Provider:
Ministry of Health
Investigator:
Jan Bureš, Daniel Jun, Marcela Kopáčová, Kamil Kuča, Martin Kuneš, Jaroslav Květina, Kamil Musílek, Stanislav Rejchrt, Ilja Tachecí, Jana Žďárová Karasová
Abstract:
Our research team will synthesise both priority compounds K203 and K027 (and reference asoxim and obidoxim) in sufficient volumes and supreme purity. Pharmacokinetics studies will set perspective therapeutic regimes (doses and rate of administration) and absorption gastrointestinal window. Bioavailability studies will determine biodistribution and effect / toxic exposition ratio in particular physiological systems. Investigation of motor functions of the upper gastrointestinal tract enables to evaluate possible therapeutic effect (against organophosphates poisoning) and possible side effects of acetylcholinesterase modulators to the gastrointestinal tract. The research team will use oesophageal impedance, pH-metry, high resolution manometry and electrogastrography. This is a priority and high impact society project.
Project duration:
1-May-2013 - 31-December-2015
