Post-doctoral fellow
+420 973 255 155
lucie.junova@fnhk.cz
ORCID: 0000-0002-1676-4932
Lucie obtained her PhD in 2009 at the Faculty of Pharmacy in Hradec Kralove, Charles University in Prague. The main goal of the doctoral thesis was chemical modification of the enzyme phosphotriesterase using biocompatible polymers, subsequent purification and biochemical characterization of prepared conjugates and their in vivo study. Second part of the thesis was focused on testing of reactivation efficacy of oxime reactivators using organophophate pesticide-inhibited butyrylcholinesterase. Currently, she works as research and development fellow at the Biomedical Research Centre, University Hospital Hradec Kralove and she is responsible for testing of cholinesterase modulatos usable as new drugs. She is an assistant professor in Department of Toxicology and Military Pharmacy, Faculty of Military Health Sciences, University of Defence at the same time.
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Ph.D. |
(2009) |
Charles University in Prague, Faculty of Pharmacy Hradec Kralove in cooperation with Department of Toxicology, Faculty of Military Health Sciences, University of Defence |
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PharmDr. |
(2009) |
Charles University in Prague, Faculty of Pharmacy Hradec Kralove |
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MSc. |
(2006) |
Charles University in Prague, Faculty of Pharmacy Hradec Kralove Diploma thesis title: Pattern of expression of TRAIL and its receptors DR5 and DcR2 in rat uterine tissues during pregnancy (supervisors: Prof. Ing. Barbora Szotáková, Ph.D., assoc.prof. Georgina Lopes Correia da Silva, thesis worked on Universidade do Porto) |
Evaluation of cholinesterase modulators
In vitro testing of inhibition and reactivation efficacy of new drugs on cholinesterases
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Since 2018 |
Assistant professor in Department of Toxicology and Military Pharmacy, Faculty of Military Health Sciences, University of Defence |
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2017 - 2018 |
Pharmacist in Hospital Pharmacy, University Hospital Hradec Kralove |
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2012 - 2017 |
Maternity leave |
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2009 - 2012 |
Pharmacist in Hospital Pharmacy, University Hospital Hradec Kralove |
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2007 - 2009 |
Translations (ENG – CZE), Contract research organization, Prague |
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2008 |
Centre de recherches du service de santé des armées), La Tronche, France (Purification, evaluation and PEGylation of organophosphate-hydrolyzing enzymes; two-month research stay) |
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2008 |
Institute for Medical Research and Occupational Health, Zagreb, Croatia (Reactivation of organophosphate-inhibited cholinesterases, two-week research stay) |
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2007 |
Centre de recherches du service de santé des armées), La Tronche, France (Purification, evaluation and PEGylation of organophosphate-hydrolyzing enzymes; three-month research stay) |
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2006 |
Universidade do Porto, Portugal (ERASMUS, 5 months – working on diploma thesis) |
Czech Pharmaceutical Society ČLS JEP - member
| 2018-2020 |
GAČR: Reaktivátory butyrylcholinesterasy pro přípravu pseudo katalytických scavengerů využitelných při intoxikacích organofosforovými sloučeninami (member of a team) |
| 2018-2021 |
Vývoj polyvalentního dekontaminačního činidla (member of a team) |
| 2017-2020 |
Ministry of Defence: Long term development plan (DZRO ZHN) (member of a team) |
| 2009 | Project DGA 08co501 – „Bioépurateurs catalytiques des neurotoxiques organophosphorés: Optimisation et Fonctionnalisation“, solved in cooperation with French workplace (C.R.S.S.A. - Centre de recherches du service de santé des armées, La Tronche, Francie); (member of a team) |
| 2008-2009 |
Institutional research plan MO0FVZ0000604 - Information support of crises management in health care (2006-2011, MO0), Ministry of Defence (Czech Republic) (member of a team) |
| 2008-2009 | NATO grant „Butyrylcholinesterase and aldoximes - bioscavengers for detoxification of organophosphates“ (CBP.EAP.CLG 983 024); (member of a team) |
| 2009 |
3rd place Prix de Pharmacie 2010 by Sanofi-Aventis and French Embassy in the Czech Republic |
| 2008 | Scholarship from French embassy for two-month research stay in France |
| 2007 | Scholarship from French embassy for three-month research stay in France |
Author or co-author of 13 scientific publications with IF
Co-autor of 1 book chapter
H-index: 9
Citations without autocitations: 200
(dated 5/2019)
KUCA, Kamil, Daniel JUN, Lucie JUNOVA, Kamil MUSILEK, Martina HRABINOVA, Jorge Alberto VALLE DA SILVA, Teodorico Castro RAMALHO, Marian VALKO, Qinghua WU, Eugenie NEPOVIMOVA a Tanos Celmar COSTA FRANCA. Synthesis, Biological Evaluation, and Docking Studies of Novel Bisquaternary Aldoxime Reactivators on Acetylcholinesterase and Butyrylcholinesterase Inhibited by Paraoxon. Molecules [online]. 2018, 23(5), 1103. ISSN 1420-3049. Dostupné z: doi:10.3390/molecules23051103
TROVASLET-LEROY, Marie, Lucie MUSILOVA, Frederique RENAULT, Xavier BRAZZOLOTTO, Jan MISIK, Ladislav NOVOTNY, Marie-Therese FROMENT, Emilie GILLON, Melanie LOIODICE, Laurent VERDIER, Patrick MASSON, Daniel ROCHU, Daniel JUN a Florian NACHON. Organophosphate hydrolases as catalytic bioscavengers of organophosphorus nerve agents. Toxicology Letters [online]. 2011, 206(1), 14–23. ISSN 0378-4274. Dostupné z: doi:10.1016/j.toxlet.2011.05.1041
JUN, Daniel, Lucie MUSILOVA, Kamil MUSILEK a Kamil KUCA. In Vitro Ability of Currently Available Oximes to Reactivate Organophosphate Pesticide-Inhibited Human Acetylcholinesterase and Butyrylcholinesterase. International Journal of Molecular Sciences [online]. 2011, 12(3), 2077–2087. ISSN 1422-0067. Dostupné z: doi:10.3390/ijms12032077
MUSILOVA, Lucie, Daniel JUN, Jiri PALECEK, Vladimir CIRKVA, Kamil MUSILEK, Martin PAAR, Martina HRABINOVA, Miroslav POHANKA a Kamil KUCA. Novel Nucleophilic Compounds with Oxime Group as Reactivators of Paraoxon-Inhibited Cholinesterases. Letters in Drug Design & Discovery [online]. 2010, 7(4), 260–264. ISSN 1570-1808. Dostupné z: doi:10.2174/157018010790945823
KOVARIK, Zrinka, Maja KATALINIC, Goran SINKO, Jiri BINDER, Ondrej HOLAS, Young-Sik JUNG, Lucie MUSILOVA, Daniel JUN a Kamil KUCA. Pseudo-catalytic scavenging: Searching for a suitable reactivator of phosphorylated butyrylcholinesterase. Chemico-Biological Interactions [online]. 2010, 187(1–3), 167–171. ISSN 0009-2797. Dostupné z: doi:10.1016/j.cbi.2010.02.023
JUN, Daniel, Lucie MUSILOVA, Miroslav POHANKA, Young-Sik JUNG, Pavel BOSTIK a Kamil KUCA. Reactivation of Human Acetylcholinesterase and Butyrylcholinesterase Inhibited by Leptophos-Oxon with Different Oxime Reactivators in Vitro. International Journal of Molecular Sciences [online]. 2010, 11(8), 2856–2863. ISSN 1422-0067. Dostupné z: doi:10.3390/ijms11082856
JUN, Daniel, Lucie MUSILOVA, Marek LINK, Melanie LOIODICE, Florian NACHON, Daniel ROCHU, Frederique RENAULT a Patrick MASSON. Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning. Chemico-Biological Interactions [online]. 2010, 187(1–3), 380–383. ISSN 0009-2797. Dostupné z: doi:10.1016/j.cbi.2010.03.017
MUSILOVA, Lucie, Kamil KUCA, Young-Sik JUNG a Daniel YUN. In vitro oxime-assisted reactivation of paraoxon-inhibited human acetylcholinesterase and butyrylcholinesterase. Clinical Toxicology [online]. 2009, 47(6), 545–550. ISSN 1556-3650. Dostupné z: doi:10.1080/15563650903058914
KUCA, Kamil, Lucie MUSILOVA, Jiri PALECEK, Vladimir CIRKVA, Martin PAAR, Kamil MUSILEK, Martina HRABINOVA, Miroslav POHANKA, Jana Zdarova KARASOVA a Daniel JUN. Novel Bisquaternary Oximes-Reactivation of Acetylcholinesterase and Butyrylcholinesterase Inhibited by Paraoxon. Molecules [online]. 2009, 14(12), 4915–4921. ISSN 1420-3049. Dostupné z: doi:10.3390/molecules14124915
KUCA, Kamil, Kamil MUSILEK, Daniel JUN, Miroslav POHANKA, Jana Zd’arova KARASOVA, Ladislav NOVOTNY a Lucie MUSILOVA. Could oxime HI-6 really be considered as „broad-spectrum" antidote? Journal of Applied Biomedicine [online]. 2009, 7(3), 143–149. ISSN 1214-0287. Dostupné z: doi:10.32725/jab.2009.016
KUCA, Kamil, Jiri CABAL, Yung Sik JUNG, Kamil MUSILEK, Ondrej SOUKUP, Daniel JUN, Miroslav POHANKA, Lucie MUSILOVA, Jana KARASOVA, Ladislav NOVOTNY a Martina HRABINOVA. Reactivation of Human Brain Homogenate Cholinesterases Inhibited by Tabun using Newly Developed Oximes K117 and K127. Basic & Clinical Pharmacology & Toxicology [online]. 2009, 105(3), 207–210. ISSN 1742-7835. Dostupné z: doi:10.1111/j.1742-7843.2009.00421.x
JUN, D., L. MUSILOVA, K. KUCA, J. KASSA a J. BAJGAR. Potency of several oximes to reactivate human acetylcholinesterase and butyrylcholinesterase inhibited by paraoxon in vitro. Chemico-Biological Interactions [online]. 2008, 175(1–3), 421–424. ISSN 0009-2797. Dostupné z: doi:10.1016/j.cbi.2008.05.004
KUCA, Kamil, Daniel JUN, Jiri CABAL a Lucie MUSILOVA. Bisquaternary oximes as reactivators of tabun-inhibited human brain cholinesterases: An in vitro study. Basic & Clinical Pharmacology & Toxicology [online]. 2007, 101(1), 25–28. ISSN 1742-7835. Dostupné z: doi:10.1111/j.1742-7843.2007.00085.x
