Professor
+420 495 832 502
Dr. Zuzana Bilkova graduated in Molecular Biology and Genetics from Charles University, Prague, Czech Republic, began her academic career at the University of Pardubice in 1993. In 2000 she defended her thesis focused on separation methods such asimmunoaffinity chromatography,she received the Ph.D. in Analytical chemistry – Immunochemistry from Faculty of Chemical Technology, University of Pardubice. In 1998 she joined the Department of Biological and Biochemical Sciences as a lecturer and in 2005 she became a head of department. In 2014, she was promoted to Professor of Clinical Immunology. Her current research interests include analytical and separation methods based on molecular recognition and modern superparamagnetic (nano)materialsused as a solid phase are combined with microfluidic systems and sensors. Her scientific expertise can be summarized: immunochemistry, bioaffinity chromatography, and structural analysis of clinically relevant biomarkers of autoimmune, neurodegenerative or cancer diseases.
| Professor | (2014) | Promotion to Professor of Clinical Immunology, lecture„Immunopatogenesisof neurodegenerative diseases; How do the conception of research activities and the education in clinical immunology influence the new biomarkers search“. |
| Assoc. Prof. | (2005) | Habilitation in Analytical Chemistry, specialization Immunochemistry, title of thesis: Analysis of proteins by affinity chromatography methods., Faculty of Chemical Technology, University of Pardubice |
| Ph.D. | (2000) | Doctoral study in Analyticalchemistry - liquid chromatography, supervisor prof. Ing. Jaromir Churáček, DrSc. (University of Pardubice, Faculty of Chemical Technology) thesis „Affinitychromatography – orientedimmobilizationof chymotrypsin“ |
| RNDr. | (1989) | Faculty of Natural Sciences - Molecular Biology and Genetics, Charles University, Prague, Czech Republic, diploma work: Strategyfor construction of genom library of S. diastaticus and isolation of gene STA, graduated with award of dean of Faculty of Natural Sciences (RNDr.) |
Structural analysis of proteins and clinically important biomarkers with own functionalized carriers with main accent to immunoanalytical methods (biomarkers of malignant diseases of hematopoetic cells, neurodegenerative and autoimmune diseases), biofunctionalization of superparamagnetic micro/nanospheres by enzymes, antibodies, microseparation and purification techniques for subsequent structural protein characterization using microfluidic systems and sensors.
| 2015 - today | Professor of clinical immunology, head of research group of immunology and immunochemistry, guarantor of study program Medical laboratory worker (bachelor) and Bioanalytics (master), supervisor of 9 doctoral students |
| 2005 - 2015 | Academic worker as Associate Professor, head of Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, head of research group of immunology and immunochemistry, guarantor of study program Clinical biology and biochemistry (bachelor) and Analysis of biological materials (master) |
| 2000 - 2005 | Academic worker as Assistant of Professor, head of research group of immunology and immunochemistry, Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice |
| 1993 - 1999 | Academic worker as Assistant, Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice |
| 2002 |
Sabbatical year at Institute Curie, Paris, France (in Dr. Jean-Louis Viovy´s research group), 1 year contract, supported by 5th European FP MicroProteoMics |
| 2003 | Short study stay at University of Constanz (research group of prof. Przibylski), Germany, in the frame of 5th European FP MicroProteoMics |
| 2002, 2004 | 2 short study stays at University of Verona (research group of prof. Righetti), Italy, in the frame of 5th European FP MicroProteoMics |
| 2008, 2009 |
2 short study staysat Indiana University, Bloomington, Indiana, USA (research groupof prof. M. Novotny) |
Czech Society of Clinical Biochemistry (from 1997)
Czech Society of Immunology (from 1996)
Committee of Czech Foundation for Universities Development (G4, F3) (2000 - 2007)
Member of Advisory board, Centre of Bioanalytical Technology, Masaryk University, Brno and Institute of Analytical Chemistry, Academy of Science, CZ (2007 - 2010)
Member of Advisory board Centreof Experimental Hematology, 1st medical Faculty, Charles University, Institute of Pathological Physiology, Prague (2007 -2011)
Member of Advisory board of doctoral program P1403 Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice (from 2005)
Member of Advisory board of doctoral program Clinical Immunology, Medical faculty Hradec Kralove, Charles University, Prague (2007 - 2009)
Advisory board of doctoral study program Pathobiochemistry and xenobiochemistry, Pharmaceutical Faculty of Charles University, Hradec Kralove(from 2007)
Member of Scientific board, Faculty of Chemical Technology, University of Pardubice(from 2010)Member of Scientific board, Medical Faculty Hradec Kralove, Charles University, Prague (from 2015)
In the period 2007 - 2010 the research group of Dr. Bilkova was the partner of the 6th FP EU project NeuroTAS:Developmentof “lab-on-chip” systemsforthe early diagnosisofneurodegenerativediseases.In the period 2008 – 2010 the research group of Dr. Bilkova was the partner of project CONTACT supported by Ministry of education AMVIS – Differential proteomic analysis of glyco- and phosphoproteins of bacteria Francisellatularensis. In the period 2009 – 2012 Dr. Bilkova has been the main coordinator of the project INCHEBIO, The innovation of special chemical and biological study programs of University of Pardubic.
In the period 2009 - 2012 the research group of Dr. Bilkova was the partner of 7th FP EU project CaMiNEMS: Integrated Micro-Nano-Opto Fluidic systems for high-content diagnosis and studies of rare cancer cells. FP7-NMP-2008-SMALL, main coordinator is Dr. Jean-Louis Viovy (France).
In the period 2009 - 2011 Dr. Bilkova has been the main coordinator of project supported by Czech Science Foundation GACR 203/09/0857: New analytical approaches for identification of proteins with significant attributes for virulence and pathogenicity of bacteria.
In the 2010 the 7th FP EU project NADINE: Nanosystems For The Early Diagnosis of Neurodegenerative Diseases.NaDiNe. FP7-NMP-2009-LARGE has started. The research group of Dr. Bilkova is a member of international research team. The maincoordinatoris Dr. JörgKötter (Denmark) and theresearchcoordinatoris Dr. Jean-Louis Viovy (France).
In the period 2012 - 2014 Dr. Bilkova will be the main coordinator of project supported by Czech Science Foundation GACR 206/12/0381, Development of ultrasensitive electrochemical immunosensors for an early detection of clinically significant biomarkers of malignant diseases.
In the period 2012 - 2015 the group of Dr. Bilkova will be the partner of the new project Molecular biotechnology school – Medical nanobiotechnology supported by European Structural Funds.
Title: Development of ultrasensitive magneto-immuno assay based on quantum dots/antibody probes for simultaneous electrochemical detection of cancer markers.
Funding: Grant Agency Czech Republic
Schedule: 2015 - 2017
Title: A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument
Funding: Horizon 2020, LOVE FOOD2MARKET
Schedule: 2016 - 2019
Title: Advanced magnetically active TiO2 nanomaterials for preparation of biological active compounds for biotechnology and medicine applications
Funding: Technology Agency of the Czech republic
Schedule: 2016 - 2017
International institutes
National oncologic institute, Bratislava, SlovakRepublic, MUDr. Jan Lakota
Virology institute of Slovak Academy of Sciences, Bratislava, SlovakRepublic, Dr. LudovítŠkultéty
Institute Curie, Paris, France (Dr. Jean-Louis Viovy) – microscale analysis of proteins, DNA, microfluidic technology
KTH, Royal Institute of technology, Schools of chemical science and engineering, Stockholm, Sweden, Dr. Johan Roerade
University Paris-Sud, Faculty of Pharmacy, Chatenay-Malabry, France, Dr. Myriam Taverna
University Hospital Basel, Department of Biomedicine, Basel, Switzerland, prof. Martin Stern
University of Duisburg-Essen, Laboratory for Molecular Neurobiology at RheinischeKliniken Essen, Essen, German, Dr. Hans Klafki
National institutes
Institute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, CZ, Dr. Daniel Horák, Dr. J. Beneš
Institute of Analytical Chemistry, CzechAcademy of Sciences, Brno, CZ, Dr. FrantišekForet
Institute of Molecular Pathology and Proteome Centre for the Study of Intracellular Parasitism of Bacteria, Purkyne Military Medical Academy, Hradec Kralove, CZ,
Institute of Physiology, Department of Analysis of Biologically Important Compounds, Czech Academy of Science, Prague, CZ, Prof. Ivan Mikšík
Centrum of neuropsychiatric studies, Alzheimer´s disease centrum, Prague, CZ, Dr. AlešBartoš, Dr. Daniela Řípová
Department of Immunology, Faculty of Medicine, PalackyUniversity, Olomouc, CZ, prof. EvženWeigl
Contipro group, R&D laboratories, DolníDobrouč, CZ, Dr. Vladimír Velebný
Since 1999 teaching and since 2002 also the guarantor of lectures:Immunology, Laboratory Methodsin Immunology, Clinical Immunology, Immunochemistry, Immunoanalyticalmethods. Since 2006, lectures Biology(partmolecular biology)and since 2008 lectures in Molecular Biology and Genetics. Currentlyguarantor2medically-oriented fields of study:Bachelor degree inMedical Laboratory Technician(accreditation ofthe Ministry of Education, Ministry of Health), Bioanalytics(accreditation ofthe Ministry of Education, Ministry of Health).
Supervisor of bachelor works (total of 21 defended) diploma works (total of25 defended)and thesis (total of 10 defended). Author and co-author of many instruction manuals for seminars and laboratory practices (Immunology, Immunochemistry, Basic microbiology), co-autor of the scripts. Chairman the the state final exams in bachelor´s and master´s and doctoral study programs.Reviewer of many bachelor, diploma, doctoral and habilitation thesis. Lecturerat the School of Molecular Biotechnology - Profession, Olomouc, 2010, 2011.Supervisorand lecturerat the Schoolof Molecular Biotechnology-Medicalnanobiotechnology, Olomouc -organization of modulesFundamentals ofcytologyStem cells, recombinant proteinsand peptides, 2013, 2014.
| 1989 | Award of the dean of Faculty of Natural Sciences for the best students of study program Molecular Biology and Genetics |
| 1998 | Award for the best poster presentation during the FONS 1998, symposium of Czech Society of Clinical Biochemistry, Karlovy Vary, Czech Republic |
2015
Thionin-modified Poly(glycidyl methacrylate) Nanospheres as Labels of Antibodies for Biosensing Applications, Zasonská B, Čadková M, Korecká L, Kovářová A, Bílková Z, Horák D., ACS Appl. Mater. Interfaces. 2015; 7; 24926 – 24931, IF = 6,723
Alkaline phosphatase labeled antibody-based electrochemical biosensor for sensitive HE4 tumor marker detection, Čadková M, Dvořáková V, Metelka R, Bílková Z, Korecká L., Electrochemistry Communications; 2015 (59) 1-4;IF: 4,287
R.M.Mohamadi, Z. Svobodova, Z. Bilkova, M. Otto, M. Taverna, S. Descroix, JL. Viovy, An integrated microfluidic chip for immunocapture, preconcentration and separation of β-amyloid peptides, Biomicrofluidics 2015 (9) 054117; IF: 3,357
Havelek, R., Cmielova, J., Kralovec, K., Bilkova Z., et al.; Specific inhibition of Wee1 kinase and Rad51 recombinase: A strategy to enhance the sensitivity of leukemic T-cells to ionizing radiation-induced DNA double-strand breaks, Biochemical and Biophysical Research Communications 2014, vol 453 (3), 569-575.
2014
Holubová L., Knotek P., Palarčík J., Čadková M., Bělina P., Vlček M., Korecká L., Bilkova Z. Magnetic microparticles post-synthetically coated by hyaluronic acid as enhanced carrier for microfluidic bioanalysis. Materials Science and Engineering C, 2014, 44, 345-351
Holubová L., Korecká L., Podzimek Š., Moravcová V., Rotková J., Ehlová T., Velebný V., Bílková Z.; Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments. Carbohydrate Polymers, 2014, 112, 271-276
Flores-Ramirez G., Jankovicova B., Bilkova Z., Miernyk J.A., Skultety L.: Identification of Coxiella burnetii surface-exposed and cell envelope associated proteins using a combined bioinformatics plus proteomics strategy. Proteomics (2014) Aug, 14(16): 1868-1881 (IF: 3.973).
Svobodova Z., Kucerova J., Autebert J., Horak D., Bruckova L., Viovy J.-L., Bilkova Z.; Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture, Electrophoresis 35(2-3), 323-329, 2014 (IF 3,3)
2013
Jankovicova B., Skultety L., Dubrovcakova M., Stern M., Bilkova Z., Lakota J.: Overlap of epitopes recognized by anti-carbonic anhydrase I IgG in patients with malignancy-related aplastic anemia-like syndrome and in patients with aplastic anemia. Immunology Letters (2013) Jun, 153 (1-2): 47-49 (IF: 2.34).
Svobodova Z., Jankovicova B., Horak D., Bilkova Z.: Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies. Journal of Analytical & Bioanalytical Techniques (2013), 4 (3): 168, doi:10.4172/2155-9872.1000168, ISSN: 2155-9872, (IF 3.89).
2012
Svobodova Z, Reza Mohamadi M, Jankovicova B, Esselmann H, Verpillot R, Otto M, Taverna M, Wiltfang J, Viovy JL, Bilkova Z.; Development of a magnetic immunosorbent for on-chip preconcentration of amyloid β isoforms: Representatives of Alzheimer's disease biomarkers. Biomicrofluidics. 2012 červen, 6(2):24126-2412612. (IF 3,37)
Horak D., Kucerova J., Korecka L., Jankovicova B., Palarcik J., Mikulasek P., Bilkova Z.; New Monodisperse Magnetic Polymer Microspheres Biofunctionalized for Enzyme Catalysis and Bioaffinity Separations, Macromolecular biosciences 2012, 12(5), str. 647-655 DOI: 10.1002/mabi.201100393. (IF 3,88)
2011
Šálek P., Korecka L., Horak D., Petrovsky E., Kovarova J., Metelka R., Bilkova Z.; Immunomagnetic sulphonated hypercrosslinked polystyrene microspheres for electrochemical detection of proteins, Journal of Materials Chemistry 2011, 21(38), str. 14783-14792. (IF 5,1)
2015
Thionin-modified Poly(glycidyl methacrylate) Nanospheres as Labels of Antibodies for Biosensing Applications, Zasonská B, Čadková M, Korecká L, Kovářová A, Bílková Z, Horák D., ACS Appl. Mater. Interfaces. 2015; 7; 24926 – 24931, IF = 6,723
Magneticbeads-basedelectrochemicalimmunosensorfor monitoring allergenic food proteins. Cadkova M, Metelka R, Holubova L, Horák D, Dvořáková V, Bílková Z, Korecká L., Anal. Biochem. 2015; 484; 4 – 8, IF = 2,219
Alkalinephosphataselabeledantibody-basedelectrochemicalbiosensorfor sensitive HE4 tumor markerdetection,Čadková M, Dvořáková V, Metelka R, Bílková Z, Korecká L., Electrochemistry Communications; 2015 (59) 1-4;IF: 4,287
Identification and characterizationof natural antibodiesagainst tau protein in anintravenousimmunoglobulinproduct, Hromadkova L., Kolarova M., Jankovicova B., Bartos A., Ricny J., Bilkova Z.,Ripova D., JournalofNeuroimmunology 289, 121-129 - IF: 2,467
R.M.Mohamadi, Z. Svobodova, Z. Bilkova, M. Otto, M. Taverna, S. Descroix, JL. Viovy, An integrated microfluidic chip for immunocapture, preconcentration and separationof β-amyloid peptides, Biomicrofluidics 2015 (9) 054117; IF: 3,357
Patent 305 599, 18. 11. 2015, Věstník 52/2015;
Čadková M., Metelka R., Holubová Ll., Horák D., Dvořáková V., Bílková Z., Korecká L.; Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins Analytical Biochemistry: Methods in theBiologicalSciences, 484 (2015) 4–8; IF: 2,305
Havelek, R., Cmielova, J., Kralovec, K., Bilkova Z., et al.; Specific inhibition of Wee1 kinase and Rad51 recombinase: A strategy to enhancethe sensitivity ofleukemic T-cells to ionizingradiation-induced DNA double-strandbreaks, Biochemical and Biophysical Research Communications 2014, vol 453 (3), 569-575.
R. Andrys, L. Zemanova, J. Lenco, Z. Bilkova, V. Wsol; Carbonyl-reducing enzymes as targets of a drug-immobili sedaffinity carrier, Chemico-Biological Interactions 234 (SI), 2015, 169-177. IF: 2,577
2014
Holubová L., Knotek P., Palarčík J., Čadková M., Bělina P., Vlček M., Korecká L., Bilkova Z. Magnetic microparticles post-synthetically coated by hyaluronic acid as enhanced carrier for microfluidic bioanalysis. Materials Science and Engineering C, 2014, 44, 345-351
Holubová L., Korecká L., Podzimek Š., Moravcová V., Rotková J., Ehlová T., Velebný V., Bílková Z.;Enhanced multiparametric hyaluronan degradation for production of molar-mass-definedfragments. CarbohydratePolymers, 2014, 112, 271-276
Flores-Ramirez G., Jankovicova B., Bilkova Z., MiernykJ.A., Skultety L.: Identification of Coxiella burnetii surface-exposed and cell envelope associated proteins using a combined bioinformatics plus proteomics strategy. Proteomics (2014) Aug, 14(16): 1868-1881 (IF: 3.973).
Haveek, R., Seifrtova, M., Kralovec, K. et al.; The effect of Amaryllidaceaealkaloidshaemanthamine and haemanthidine on cell cycleprogression and apoptosis in p53-negativehumanleukemicJurkatcells, Phytomedicine 21 (4), 479-490, 2014.
J.Kucerova, Z. Svobodová, P. Knotek, J. Palarčík, M. Vlček, M. Kincl, D. Horák, J. Autebert, J.-L.Viovy, Z. Bilkova. PE Gylation of magnetic poly(glycidylmethacrylate) microparticles for microfluidicbio assays. Materials Science and Engineering C: Materials for biological applications 40, 308-315, 2014 (IF 2.40)
Bílková Z.,New Formats of Antigen-BindingMolecules, Chemické listy108 (3), 198-204, 2014
Svobodova Z., Kucerova J., Autebert J., Horak D., Bruckova L., Viovy J.-L., Bilkova Z.; Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture, Electrophoresis 35(2-3), 323-329, 2014 (IF 3,3)
2013
Skarydova L., Andrys R., Holubova L., Stambergova H., Knavova J., Wsol V., Bilkova Z.;Efficientisolationofcarbonylreducingenzymesusingaffinityapproachwithanticancer drug Oracin as a specific ligand, J. Sep. Sci. Jan. 2013, DOI: 10.1002/jssc.201201141. (IF 2,53)
Jankovicova B., Skultety L., Dubrovcakova M., Stern M., Bilkova Z., Lakota J.: Overlapofepitopesrecognized by anti-carbonicanhydrase I IgG in patientswithmalignancy-relatedaplasticanemia-like syndrome and in patientswithaplasticanemia. ImmunologyLetters (2013) Jun, 153 (1-2): 47-49 (IF: 2.34).
Svobodova Z., Jankovicova B., Horak D., Bilkova Z.: Dot-ELISA Affinity Test: AnEasy, Low-CostMethod to EstimateBindingActivityofMonoclonalAntibodies. JournalofAnalytical&BioanalyticalTechniques (2013), 4 (3): 168, doi:10.4172/2155-9872.1000168, ISSN: 2155-9872, (IF 3.89).
Horak D., Svobodova Z., Autebert J., Coudert B., Plichta Z., Kralovec K., Bilkova Z.; Viovy J.-L., Albumin-coatedmonodispersemagneticpoly(glycidylmethacrylate) microsphereswithimmobilizedantibodies: Application to thecaptureofepithelialcancercells, J Biomed Mater Res 2013, 101A(1), str. 23-32. (IF 2,625)
2012
Svobodova Z, RezaMohamadi M, Jankovicova B, Esselmann H, Verpillot R, Otto M, Taverna M, Wiltfang J, Viovy JL, Bilkova Z.; Developmentof a magneticimmunosorbentfor on-chippreconcentrationof amyloid β isoforms: RepresentativesofAlzheimer'sdiseasebiomarkers.Biomicrofluidics. 2012 červen, 6(2):24126-2412612. (IF 3,37)
Horak D., Kucerova J., Korecka L., Jankovicova B., Palarcik J., Mikulasek P., Bilkova Z.; New MonodisperseMagnetic Polymer MicrospheresBiofunctionalizedfor Enzyme Catalysis and BioaffinitySeparations, Macromolecularbiosciences 2012, 12(5), str. 647-655 DOI: 10.1002/mabi.201100393. (IF 3,88)
2011
Šálek P., Korecka L., Horak D., Petrovsky E., Kovarova J., Metelka R., Bilkova Z.;Immunomagneticsulphonatedhypercrosslinked polystyrene microspheresforelectrochemicaldetectionofproteins, JournalofMaterialsChemistry 2011, 21(38), str. 14783-14792. (IF 5,1)
